gga-miR-20b-5p inhibits infectious bursal disease virus replication via targeting Netrin 4

Zixian Chen; Mei Leng; Zhishan Liang; Puduo Zhu; Sheng Chen; Qingmei Xie; Feng Chen; Wencheng Lin

doi:10.1016/j.vetmic.2023.109676

gga-miR-20b-5p inhibits infectious bursal disease virus replication via targeting Netrin 4

Vet Microbiol. 2023 Apr:279:109676. doi: 10.1016/j.vetmic.2023.109676. Epub 2023 Feb 4.

Authors

Zixian Chen¹, Mei Leng¹, Zhishan Liang¹, Puduo Zhu¹, Sheng Chen¹, Qingmei Xie¹, Feng Chen², Wencheng Lin³

Affiliations

¹ Guangdong Provincial Animal Virus Vector Vaccine Engineering Technology Research Center & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction of Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China.
² Guangdong Provincial Animal Virus Vector Vaccine Engineering Technology Research Center & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction of Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: fengch@scau.edu.cn.
³ Guangdong Provincial Animal Virus Vector Vaccine Engineering Technology Research Center & Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding & Key Laboratory of Chicken Genetics, Breeding and Reproduction of Ministry of Agriculture, College of Animal Science, South China Agricultural University, Guangzhou 510642, PR China. Electronic address: wenchenglin@scau.edu.cn.

PMID: 36796296
DOI: 10.1016/j.vetmic.2023.109676

Abstract

MicroRNAs (miRNAs) involved host-virus interaction, affecting the replication or pathogenesis of several viruses. Frontier evidences suggested that miRNAs play essential roles in infectious bursal disease virus (IBDV) replication. However, the biological function of miRNAs and the underlying molecular mechanisms are still unclear. Here, we reported that gga-miR-20b-5p acted as a negative factor affecting IBDV infection. We found that gga-miR-20b-5p was significantly up-regulated during IBDV infection in host cells, and that gga-miR-20b-5p effectively inhibited IBDV replication via targeting the expression of host protein netrin 4 (NTN4). In contrast, inhibition of endogenous miR-20b-5p markedly facilitated viral replication associated with enhancing NTN4 expression. Collectively, these findings highlight a crucial role of gga-miR-20b-5p in IBDV replication.

Keywords: IBDV; MiR-20b-5p; Netrin 4.

MeSH terms

Animals
Chickens
Host Microbial Interactions
Infectious bursal disease virus* / genetics
MicroRNAs* / metabolism
Netrins
Virus Replication / physiology

Substances

MicroRNAs
Netrins