Smartphone-based chemiluminescence detection of aflatoxin B1 via labelled and label-free dual sensing systems

Junning Ma; Yue Guan; Fuguo Xing; Yan Wang; Xu Li; Qiang Yu; Xiaohua Yu

doi:10.1016/j.foodchem.2023.135654

Smartphone-based chemiluminescence detection of aflatoxin B₁ via labelled and label-free dual sensing systems

Food Chem. 2023 Jul 1:413:135654. doi: 10.1016/j.foodchem.2023.135654. Epub 2023 Feb 10.

Authors

Junning Ma¹, Yue Guan², Fuguo Xing³, Yan Wang², Xu Li¹, Qiang Yu⁴, Xiaohua Yu⁴

Affiliations

¹ Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
² College of Food Science and Technology, Zhejiang University of Technology, Hangzhou 310014, China.
³ Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs/Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: xingfuguo@caas.cn.
⁴ Qingdao Tianxiang Foods Group Co., Ltd, Qingdao 266737, China.

PMID: 36796268
DOI: 10.1016/j.foodchem.2023.135654

Abstract

To develop a sensing platform for onsite determination of AFB₁ in foodstuffs, we developed smartphone-based chemiluminescence detection of AFB₁ via labelled and label-free dual modes. The labelled mode was characteristic of double streptavidin-biotin mediated signal amplification, obtaining limit of detection (LOD) of 0.04 ng/mL in the linear range of 1-100 ng/mL. To reduce the complexity in the labelled system, a label-free mode based on both split aptamer and split DNAzyme was fabricated. A satisfactory LOD of 0.33 ng/mL was generated in the linear range of 1-100 ng/mL. Both labelled and label-free sensing systems achieved outstanding recovery rate in AFB₁-spiked maize and peanut kernel samples. Finally, two systems were successfully integrated into smartphone-based portable device based on custom-made components and android application, achieving comparable AFB₁ detection ability to a commercial microplate reader. Our systems hold huge potential for AFB₁ onsite detection in food supply chain.

Keywords: Chemiluminescence; Label-free; Smartphone; Split DNAzyme; Split aptamer.

MeSH terms

Aflatoxin B1 / analysis
Aptamers, Nucleotide*
Biosensing Techniques*
Food Contamination / analysis
Limit of Detection
Luminescence
Smartphone

Substances

Aflatoxin B1
Aptamers, Nucleotide