Multiplex arrays designed for enzyme-linked immunosorbent assays (ELISAs) are robust and cost-effective for profiling biomarkers. Identification of relevant biomarkers in biological matrices or fluids helps in the understanding of disease pathogenesis. Here, we describe a sandwich ELISA-based multiplex assay to assess growth factor and cytokine levels in cerebrospinal fluid (CSF) samples derived from multiple sclerosis patients, amyotrophic lateral sclerosis patients, and control subjects without any neurological disorder. Results indicate that multiplex assay designed for the sandwich ELISA method is a unique, robust, and cost-effective method for profiling growth factors and cytokines present in CSF samples.
Keywords: Amyotrophic lateral sclerosis (ALS); Biomarkers; Cerebrospinal fluid samples (CSFs); Cytokines; Growth factors; Multiple sclerosis (MS); Multiplex array; Sandwich ELISA.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.