Effect of butylated hydroxyanisole on the metabolism of N-nitrosodi-n-butylamine and N-nitrosobutyl(4-hydroxybutyl)amine by rat hepatic S9 preparations in vitro

IARC Sci Publ. 1987:(84):183-4.

Abstract

N-Nitrosodi-n-butylamine (NDBA), the NADPH generating system and various concentrations of butylated hydroxyanisole (BHA) added to rat hepatic S9 fractions resulted in a significant drop (30-50%) in N-nitrosobutyl(4-hydroxybutyl)amine (NBHBA) formation and a consequent rise in the amount of substrate recovered unchanged. When NBHBA and NAD+ were incubated with BHA and S9 fractions, the amount of N-nitrosobutyl(3-carboxypropyl)amine (NBCPA) was decreased by 20-40%, and the amount of unmetabolized NBHBA increased.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Butylated Hydroxyanisole / pharmacology*
  • Butylhydroxybutylnitrosamine / metabolism*
  • Cytochrome P-450 Enzyme System / metabolism
  • Liver / drug effects
  • Liver / metabolism*
  • Nitrosamines / metabolism*
  • Nitrosamines / pharmacokinetics
  • Rats

Substances

  • Nitrosamines
  • Butylated Hydroxyanisole
  • Butylhydroxybutylnitrosamine
  • butyl(3-carboxypropyl)nitrosamine
  • dibutylnitrosamine
  • Cytochrome P-450 Enzyme System