Region-resolved multi-omics of the mouse eye

Hang Xiang; Bohan Zhang; Yunzhi Wang; Ning Xu; Fan Zhang; Rongkui Luo; Minbiao Ji; Chen Ding

doi:10.1016/j.celrep.2023.112121

Region-resolved multi-omics of the mouse eye

Cell Rep. 2023 Feb 28;42(2):112121. doi: 10.1016/j.celrep.2023.112121. Epub 2023 Feb 14.

Authors

Hang Xiang¹, Bohan Zhang², Yunzhi Wang¹, Ning Xu¹, Fan Zhang¹, Rongkui Luo³, Minbiao Ji⁴, Chen Ding⁵

Affiliations

¹ State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Institute of Biomedical Sciences, Human Phenome Institute, Zhongshan Hospital, Fudan University, Shanghai 200433, China.
² State Key Laboratory of Surface Physics and Department of Physics, Human Phenome Institute, Multiscale Research Institute of Complex Systems, Academy for Engineering and Technology, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai 200433, China.
³ Department of Pathology, Zhongshan Hospital Fudan University, Shanghai, China.
⁴ State Key Laboratory of Surface Physics and Department of Physics, Human Phenome Institute, Multiscale Research Institute of Complex Systems, Academy for Engineering and Technology, Key Laboratory of Micro and Nano Photonic Structures (Ministry of Education), Fudan University, Shanghai 200433, China. Electronic address: minbiaoj@163.com.
⁵ State Key Laboratory of Genetic Engineering and Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Institute of Biomedical Sciences, Human Phenome Institute, Zhongshan Hospital, Fudan University, Shanghai 200433, China. Electronic address: chend@fudan.edu.cn.

PMID: 36790928
DOI: 10.1016/j.celrep.2023.112121

Abstract

The eye is a complex organ consisting of multiple compartments with unique and specialized properties, and small disturbances in one eye region can result in impaired vision and blindness. Although there have been advancements in ocular research, the hierarchical molecular network in region-wide resolution, indicating the division of labor and crosstalk among different eye regions, is not yet comprehensively illuminated. Here, we present an atlas of region-resolved proteome and lipidome of mouse eye. Multiphoton microscopy-guided laser microdissection combined with in-depth label-free proteomics identifies 13,536 proteins across various mouse eye regions. Further integrative analysis of spectral imaging, label-free proteome, and imaging mass spectrometry of the lipidome and phosphoproteome reveals distinctive molecular features, including proteins and lipids of various anatomical mouse eye regions. These deposited datasets and our open proteome server integrating all information provide a valuable resource for future functional and mechanistic studies of mouse eye and ocular disease.

Keywords: CP: Cell biology; MALDI-TOF imaging; lipidome; mouse eye; multimodal multiphoton; region-resolved proteome.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Eye
Face
Mice
Multiomics*
Proteome* / analysis

Substances

Proteome