Plants are always better choice for treatment of disease in contrast to synthetic agents due to their less toxicity, and free availability with minor limitations of identifications, purity and potency which should be addressed. The objective of current study was to explore the anti-inflammatory effect of methanol extract of Yucca elephantipes roots by using oxidative burst assay and carrageen an induced rat paw edema. GC-MS analysis was carried for the determination of ani-inflammatory potential of fatty acids and other phytochemicals present in Yucca elephantipes roots. Among fifteen detected compounds trans-13-octadecenoic acid, n-hexadecanoic acid and 4-hydroxy benzoic acid were found as 84.21%, 5.21% and 2.17% respectively. Oxidative burst assay showed anti-inflammatory potential of Yucca elephantipes roots 74.58±0.32% with IC50 of 15.3±2.2μg/mL as compared to Ibuprofen with percentage of inhibition 73.20±0.17% and IC50 was 11.2±0.98μg/mL. Fortunately, less than 8g/kg dose of the Yucca elephantipes roots found safe in albino rats. Interestingly, in-vivo carrageen an induced paw edema method proved its anti-inflammatory potential at dose 100 and 200mg/kg in albino rats. Conclusively, 200mg/kg dose of Yucca elephantipes roots extract was optimized for 88.89±0.015% anti-inflammatory effect which can be considered most potent, safe and better alternative of synthetic drugs.