More negative charges on roots enhanced manganese(II) uptake in leguminous and non-leguminous poaceae crops

Abstract

Background: Manganese (Mn) is an essential micronutrient for plants, whereas excess Mn(II) in soils leads to its toxicity to crops. Mn(II) is adsorbed onto plant roots from soil solution and then absorbed by plants. Root charge characteristics should affect Mn(II) toxicity to crops and Mn(II) uptake by the roots of the crops. However, the differences in the effects of root surface charge on the uptake of Mn(II) among various crop species are not well understood.

Results: The roots of nine legumes and six non-legume poaceae were obtained by hydroponics and the streaming potential method and spectroscopic analysis were used to measure the zeta potentials and functional groups on the roots, respectively. The results indicate that the exchangeable Mn(II) adsorbed by plant roots was significantly positively correlated with the Mn(II) accumulated in plant shoots. Legume roots carried more negative charges and functional groups than non-legume poaceae roots, which was responsible for the larger amounts of exchangeable Mn(II) on legume roots in 2 h and the Mn(II) accumulated in their shoots in 48 h. Coexisting cations, such as Ca²⁺ and Mg²⁺ , were most effective in decreasing Mn(II) taken up by roots and accumulated in shoots than K⁺ and Na⁺ . This was because Ca²⁺ and Mg²⁺ could compete with Mn(II) for active sites on plant roots more strongly compared to K⁺ and Na⁺ .

Conclusion: The root surface charge and functional groups are two important factors influencing Mn(II) uptake by roots and accumulation in plant shoots. © 2023 Society of Chemical Industry.

Keywords: Mn(II) chemical forms; Mn(II) sorption; Mn(II) uptake; functional groups; negative charge; plant roots.