Integration of solid-phase microextraction and surface-enhanced Raman spectroscopy for in-vivo screening of polybrominated diphenyl ether

Mengping Zhang; Xiao Meng; Nianlu Li; Wei Zou; Haiyan Wei; Ranran Liu; Yaxin Sun; Wenwen Chen; Jingcheng Cui; Cuijuan Wang

doi:10.1016/j.saa.2023.122476

Integration of solid-phase microextraction and surface-enhanced Raman spectroscopy for in-vivo screening of polybrominated diphenyl ether

Spectrochim Acta A Mol Biomol Spectrosc. 2023 May 15:293:122476. doi: 10.1016/j.saa.2023.122476. Epub 2023 Feb 10.

Authors

Mengping Zhang¹, Xiao Meng¹, Nianlu Li¹, Wei Zou¹, Haiyan Wei¹, Ranran Liu¹, Yaxin Sun², Wenwen Chen¹, Jingcheng Cui³, Cuijuan Wang⁴

Affiliations

¹ Physical and Chemical Laboratory, Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250000, PR China.
² School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, PR China.
³ School of Municipal and Environmental Engineering, Shandong Jianzhu University, Jinan 250101, Shandong, China. Electronic address: cuijingcheng19@sdjzu.edu.cn.
⁴ Physical and Chemical Laboratory, Shandong Academy of Occupational Health and Occupational Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan 250000, PR China. Electronic address: wangcuijuan@sdfmu.edu.cn.

PMID: 36787678
DOI: 10.1016/j.saa.2023.122476

Abstract

The monitoring of polybrominated diphenyl ethers (PBDEs) is of great significance owing to their high persistence, bioaccumulation, and toxicity to humans and animals. In this study, a sensitive and reproducible probe that integrates solid-phase microextraction and surface-enhanced Raman spectroscopy (SPME-SERS) was developed for screening PBDEs in multiphase specimens, including live fish, water, and electrical products. A roughed Cu fiber with an Ag layer was fabricated with dual functions. BDE-15 was readily extracted and detected on the SPME-SERS probe consisting of propanethiol-modified Ag nanoplates on a Cu wire. A clear linear relationship (R² = 0.988) was established between the SERS intensity at 782 cm^-1 and the logarithmic concentrations (from 100 ppb to 100 ppm), with a detection limit of 15 ppb. This proposed method enables continuous in vivo monitoring in fish without complicated pretreatments. The results obtained by this SPME-SERS approach were validated by high-performance liquid chromatography and showed good agreement. This "extracting and detecting" SPME-SERS method provides a potential tool to monitor the occurrence, formation, and migration of PBDEs.

Keywords: BDE-15; In vivo detection; Integrated analysis method; Solid-phase microextraction; Surface-enhanced Raman Scattering.

MeSH terms

Animals
Halogenated Diphenyl Ethers* / analysis
Humans
Solid Phase Microextraction* / methods
Spectrum Analysis, Raman / methods
Water

Substances

Halogenated Diphenyl Ethers
Water