Preparation of Giant Vesicles with Mixed Single-Tailed and Double-Tailed Lipids

Lauren A Lowe; Anna Wang

doi:10.1007/978-1-0716-2954-3_6

Preparation of Giant Vesicles with Mixed Single-Tailed and Double-Tailed Lipids

Methods Mol Biol. 2023:2622:71-85. doi: 10.1007/978-1-0716-2954-3_6.

Authors

Lauren A Lowe^{1

2}, Anna Wang^{3

4}

Affiliations

¹ School of Chemistry, UNSW Sydney, Sydney, NSW, Australia.
² Australian Centre for Astrobiology, UNSW Sydney, Sydney, NSW, Australia.
³ School of Chemistry, UNSW Sydney, Sydney, NSW, Australia. anna.wang@unsw.edu.au.
⁴ Australian Centre for Astrobiology, UNSW Sydney, Sydney, NSW, Australia. anna.wang@unsw.edu.au.

PMID: 36781751
DOI: 10.1007/978-1-0716-2954-3_6

Abstract

Giant vesicles are model membrane systems that can be characterized with microscopy. Whereas most giant synthetic vesicles are created with a single phospholipid species, vesicles with mixed membrane compositions, including single-tailed and double-tailed lipids, serve as more accurate models of biological membranes and also have applications in the origins of life and drug delivery fields. Here we describe several approaches that can be used to create giant vesicles with mixed lipid compositions.

Keywords: Double-chain; Fatty acids; GUVs; Lipid bilayers; Membranes; Phospholipids; Single-chain; Vesicles.

MeSH terms

Cell Membrane
Lipid Bilayers
Microscopy*
Phospholipids*
Unilamellar Liposomes

Substances

Phospholipids
Unilamellar Liposomes
Lipid Bilayers