Microbial Genome Sequencing and Assembly Using Nanopore Sequencers

Makoto Taniguchi; Kazuma Uesaka

doi:10.1007/978-1-0716-2996-3_3

Microbial Genome Sequencing and Assembly Using Nanopore Sequencers

Methods Mol Biol. 2023:2632:31-39. doi: 10.1007/978-1-0716-2996-3_3.

Authors

Makoto Taniguchi^{1

2}, Kazuma Uesaka³

Affiliations

¹ Oral Microbiome Center, Taniguchi Dental Clinic, Kagawa, Japan. makoto@tani8020.jp.
² Genome Read Inc., Kagawa, Japan. makoto@tani8020.jp.
³ The Center for Gene Research, Nagoya University, Aichi, Japan.

PMID: 36781719
DOI: 10.1007/978-1-0716-2996-3_3

Abstract

Microbial genomes are typically several million base pairs in length and are relatively easy to sequence and assemble into a single chromosome, given the advances in long-read sequencing platforms such as that of Oxford Nanopore Technologies. This chapter describes the experimental as well as computational steps in the sequencing and assembly of microbial genomes.

Keywords: Complete genome; Genome assembly; Microbial genomes; Nanopore sequencing.

MeSH terms

Genome, Bacterial
Genome, Microbial
High-Throughput Nucleotide Sequencing
Nanopores*
Sequence Analysis, DNA