Tubulin-Based Polymorphism (TBP) in Plant Genotyping

Methods Mol Biol. 2023:2638:387-401. doi: 10.1007/978-1-0716-3024-2_28.

Abstract

Tubulin-based polymorphism (TBP) is an intron length polymorphism (ILP) method widely applicable to any plant species and particularly suitable for a first and rapid classification of any plant genome. It is based on the selective, polymerase chain reaction (PCR)-based amplification of the two introns present at conserved positions within the coding sequences of plant β-tubulin genes. Amplification releases a simple yet distinctive genomic profile.

Keywords: DNA fingerprinting; DNA polymorphism; Introns; Molecular markers; Tubulin.

MeSH terms

  • Genes, Plant
  • Genotype
  • Introns / genetics
  • Plants / genetics
  • Polymorphism, Genetic*
  • Tubulin* / genetics

Substances

  • Tubulin