Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Yang Zhou; Huitang Pan

doi:10.1007/978-1-0716-3024-2_11

Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Methods Mol Biol. 2023:2638:165-171. doi: 10.1007/978-1-0716-3024-2_11.

Authors

Yang Zhou¹, Huitang Pan²

Affiliations

¹ Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China.
² Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, China. htpan@bjfu.edu.cn.

PMID: 36781641
DOI: 10.1007/978-1-0716-3024-2_11

Abstract

Specific length amplified fragment sequencing (SLAF-seq) technology is a simplified genome sequencing technology based on next-generation sequencing. SLAF-seq technology has several distinguishing characteristics: 1. Deep sequencing to ensure accuracy of genotyping; 2. Effectively reduce sequencing costs; 3. Pre-designed simplified representation scheme to optimize marker efficiency; 4. Doubled barcode system for large populations. The advantages and technical process of SLAF-seq are described briefly with summarized results for the application of SLAF-seq in development of molecular markers, construction of high-density genetic map and gene mapping in ornamental plants. Finally, the difficulties and prospects of this method are discussed in application.

Keywords: Genotyping; Molecular markers; SLAF; SNP.

MeSH terms

Chromosome Mapping / methods
High-Throughput Nucleotide Sequencing* / methods
Polymorphism, Single Nucleotide
Quantitative Trait Loci*
Sequence Analysis, DNA / methods