Double Digest Restriction-Site Associated DNA Sequencing (ddRADseq) Technology

Abstract

Double digest restriction-site associated DNA sequencing (ddRADseq) technology combines genome reduced representation by digestion with two restriction enzymes and next generation sequencing (NGS) to obtain thousands of markers (SNP, SSR, and InDels) and genotype tens to hundreds of samples simultaneously. In this chapter, we describe a 96-plex derived ddRADseq protocol that can be set up to obtain different depth of coverage per locus and can be exploited to model and non-model plant species.

Keywords: Genotyping by sequencing; InDels; Molecular markers; Next generation sequencing; RADseq; Reduced representation libraries; SNP; SSR.