Photodynamic activity enhanced by in situ biosynthetic BC/CQDs@PCN-224 membranes through FRET strategy

Abstract

Porphyrin-based metal organic frameworks (MOFs) with efficient bactericidal performance have increasingly attracted attention in photodynamic inactivation materials. However, low reactive oxygen species (ROS) yield and drug residue hazards of current porphyrin-MOFs materials lead to unsatisfactory clinical therapeutic effects. In this paper, carbon quantum dots (CQDs) were encapsulated into PCN-224, which enhanced the photodynamic activity of the MOFs through fluorescence resonance energy transfer (FRET) process. Singlet oxygen (¹O₂) detection confirmed that the photodynamic activity of CQDs-doped PCN-224 (CQDs@PCN-224) was enhanced than that of pristine PCN-224 under illumination. Furthermore, the CQDs@PCN-224 were firmly embedded into bacterial cellulose (BC) nanofibrous membranes by using an eco-friendly biosynthetic approach, efficiently preventing MOFs leakage during use. The results of bactericidal assays demonstrated that BC/CQDs@PCN-224 membrane with higher photodynamic activity causes more severe disruption to bacterial structure and possesses better antibacterial efficiency (>99.99 % reduction of both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli O157:H7 within 30 min) than BC/PCN-224 membrane under visible light illumination (500 W, 15 cm height, λ ≥ 420 nm). In addition, the biosynthesized BC/CQDs@PCN-224 membrane showed good hemocompatibility and low cytotoxicity, revealing that the BC- and MOFs-based material with enhanced PDI efficiency and satisfying safety has great potential in medical fields.

Keywords: BC membrane; Carbon quantum dots; FRET; PCN-224; Photodynamic inactivation.