Soy protein hydrolysates (SPHs) are prepared from soybean meal using commercially available protease enzymes and acid/alkali treatment. The antioxidant properties of SPHs were evaluated by measuring headspace oxygen consumption and conjugated diene formation in oil-in-water (O/W) emulsions. In addition, volatile profiles were analyzed for the heated mixture of SPHs and the coconut oil (SPHCO). Total amino acid content was the highest in double proteases. SPHs prepared from enzymes acted as better antioxidants than those prepared from acid/alkali treatments in O/W emulsions. SPHs prepared from double proteases generated the highest amounts of total volatiles and nitrogen-containing compounds in SPHCO. 2,3-Dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, 2-methyl-butanal, benzeneacetaldehyde, and 2,6-dimethylpyrazine were the major volatiles in SPHCO. Enzymatic SPHs act as natural antioxidants in the O/W emulsion matrix, and thermal reaction products from SPHCO may contribute to the production of a unique volatile flavor in plant protein-based foods.
Supplementary information: The online version contains supplementary material available at 10.1007/s10068-022-01189-7.
Keywords: Antioxidant; Coconut oil; Reaction volatile; Soy protein hydrolysate.
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