Simultaneous extraction and determination of alkaloids and organic acids in Uncariae Ramulas Cum Unicis by vortex-assisted matrix solid phase dispersion extraction coupled with UHPLC-MS/MS

Xianjun Xu; Jiake Wen; Shuangqi Wang; Jia Hao; Kunze Du; Shiming Fang; Jun He; Jin Li; Yanxu Chang

doi:10.3389/fchem.2023.1100150

Simultaneous extraction and determination of alkaloids and organic acids in Uncariae Ramulas Cum Unicis by vortex-assisted matrix solid phase dispersion extraction coupled with UHPLC-MS/MS

Front Chem. 2023 Jan 25:11:1100150. doi: 10.3389/fchem.2023.1100150. eCollection 2023.

Authors

Xianjun Xu¹, Jiake Wen², Shuangqi Wang², Jia Hao², Kunze Du², Shiming Fang², Jun He², Jin Li², Yanxu Chang^{2

3}

Affiliations

¹ Wuyishan Institute of biology, Nanping, Fujian, China.
² State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
³ Haihe Laboratory of Modern Chinese Medicine, Tianjin, China.

Abstract

A simple and efficient vortex-assisted matrix solid phase dispersion with a ultra-high-performance liquid chromatography-triple quadrupole mass spectrometer (VA-MSPD-UHPLC-MS/MS) was applied for simultaneous extraction and determination of seven alkaloids and three organic acids from Uncariae Ramulas Cum Unicis. The optimal extraction conditions of the target components were obtained by Box-Behnken design (BBD) combined with response surface methodology (RSM). The results of the method validation showed that this analytical method displayed good linearity with a correlation coefficient (r) no lower than 0.9990. The recoveries of ten active ingredients from Uncariae Ramulas Cum Unicis ranged from 95.9% to 103% (RSD ≤ 2.77%). The RSDs of intra-day and inter-day precisions were all below 2.97%. The present method exhibited not only lower solvent and sample usage, but also shorter sample processing and analysis time. Consequently, the developed VA-MSPD-UHPLC-MS/MS method could be successfully and effectively used for the extraction and analysis of ten active components from Uncariae Ramulas Cum Unicis.

Keywords: UHPLC-MS/MS; Uncariae Ramulas Cum Unicis; response surface methodology; silica; vortex-assisted matrix solid phase dispersion.

Grants and funding

This study was supported by the Science and Technology Program of Fujian (2020R1008001), the Science and Technology Program of Tianjin (21ZYJDJC00080), and the Special Program of Talents Development for Excellent Youth Scholars in Tianjin.