Dhx15 regulates zebrafish intestinal development through the Wnt signaling pathway

Jinpeng Yao; Yuanhua Cai; Zhixiang Chen; Xuechun Wang; Xiaolin Lai; Lili Pan; Yang Li; Shaoyuan Wang

doi:10.1016/j.ygeno.2023.110578

Dhx15 regulates zebrafish intestinal development through the Wnt signaling pathway

Genomics. 2023 Mar;115(2):110578. doi: 10.1016/j.ygeno.2023.110578. Epub 2023 Feb 10.

Authors

Jinpeng Yao¹, Yuanhua Cai², Zhixiang Chen¹, Xuechun Wang¹, Xiaolin Lai², Lili Pan², Yang Li², Shaoyuan Wang³

Affiliations

¹ Union Clinical Medical Colleges, Fujian Medical University, Fuzhou, China.
² Union Clinical Medical Colleges, Fujian Medical University, Fuzhou, China; Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou, China.
³ Union Clinical Medical Colleges, Fujian Medical University, Fuzhou, China; Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou, China. Electronic address: shaoyuanwang@fjmu.edu.cn.

PMID: 36775210
DOI: 10.1016/j.ygeno.2023.110578

Abstract

DEAH-box helicase 15 (DHX15) is ATP-dependent RNA helicase which is known for its role in RNA metabolism. Recent studies reported DHX15 involves in the intestinal immunity. However, the role of DHX15 (or RNA helicase) in intestinal development is poorly understood. Here, we revealed an unidentified role for dhx15 in regulating zebrafish intestinal development. We found the profound intestinal defects in dhx15 knockout zebrafish. Decreased proliferation and increased apoptosis of the intestine cells were observed when dhx15 were deleted. Further RNA genome wide analysis and qRT-PCR analysis showed the Wnt signaling pathway is down-regulated in the dhx15 knockout zebrafish. Thus, we concluded that dhx15 regulates zebrafish intestinal development through the Wnt signaling pathway. Here, we provided new insights into the role of dhx15 in intestinal development beyond its well-characterized role in intestinal immunity.

Keywords: Intestinal development; The Wnt signal pathway; Zebrafish; dhx15.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
RNA / metabolism
RNA Helicases / genetics
Wnt Signaling Pathway*
Zebrafish* / genetics

Substances

RNA
RNA Helicases
dhx15 protein, zebrafish