Enhanced profiling and quantification of ginsenosides from mountain-cultivated ginseng and comparison with garden-cultivated ginseng

Hua Qu; Jing Wang; Changliang Yao; Xuemei Wei; Yisong Wu; Mengzhen Cheng; Xin He; Jiayuan Li; Wenlong Wei; Jianqing Zhang; Qirui Bi; De-An Guo

doi:10.1016/j.chroma.2023.463826

Enhanced profiling and quantification of ginsenosides from mountain-cultivated ginseng and comparison with garden-cultivated ginseng

J Chromatogr A. 2023 Mar 15:1692:463826. doi: 10.1016/j.chroma.2023.463826. Epub 2023 Jan 24.

Authors

Hua Qu¹, Jing Wang², Changliang Yao³, Xuemei Wei³, Yisong Wu³, Mengzhen Cheng³, Xin He¹, Jiayuan Li³, Wenlong Wei³, Jianqing Zhang³, Qirui Bi³, De-An Guo⁴

Affiliations

¹ School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China; National Engineering Research Center of TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
² National Engineering Research Center of TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China.
³ National Engineering Research Center of TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
⁴ School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, China; National Engineering Research Center of TCM Standardization Technology, Shanghai Research Center for Modernization of Traditional Chinese Medicine, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; School of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, China. Electronic address: daguo@simm.ac.cn.

PMID: 36774914
DOI: 10.1016/j.chroma.2023.463826

Abstract

Panax ginseng can be generally divided into mountain-cultivated ginseng (MCG) and garden-cultivated ginseng (GCG). The market price of MCG is significantly higher than that of GCG. However, the chemical compositions of MCG and the differences from GCG remained unclear. In this study, an integrated strategy combing an offline two-dimensional liquid chromatography separation, LTQ-orbitrap dual mode acquisition, and Q-trap full quantification/quasi-quantification was proposed to explore and compare the chemical compositions of MCG. Consequently, 559 ginsenosides were characterized, among which 437 ginsenosides were in-depth characterized with α-chain and β-chain annotated. Subsequently, enhanced quantification of 213 ginsenosides was conducted in 57 batches of MCG and GCG. Ginsenosides were found more abundant in MCG than GCG. In addition, 25-year-old MCG could be distinctly differentiated from 15/20-year-old MCG. This strategy facilitated the enhanced profiling and comparison of ginsenosides, improved the quality control tactics of MCG and provided a reference approach for other ginseng related products.

Keywords: Chemical characterization; Ginsenosides; Mountain-cultivated ginseng; Precursor ion list; Quasi-quantification.

MeSH terms

Chromatography, High Pressure Liquid / methods
Gardens
Ginsenosides* / analysis
Panax* / chemistry
Quality Control

Substances

Ginsenosides