Capsaicin-loaded alginate nanoparticles embedded polycaprolactone-chitosan nanofibers as a controlled drug delivery nanoplatform for anticancer activity

Azin Rashidy Ahmady; Atefeh Solouk; Saeed Saber-Samandari; Somaye Akbari; Hadi Ghanbari; Bogumil E Brycki

doi:10.1016/j.jcis.2023.01.139

Capsaicin-loaded alginate nanoparticles embedded polycaprolactone-chitosan nanofibers as a controlled drug delivery nanoplatform for anticancer activity

J Colloid Interface Sci. 2023 May 15:638:616-628. doi: 10.1016/j.jcis.2023.01.139. Epub 2023 Jan 31.

Authors

Azin Rashidy Ahmady¹, Atefeh Solouk², Saeed Saber-Samandari³, Somaye Akbari⁴, Hadi Ghanbari⁵, Bogumil E Brycki⁶

Affiliations

¹ Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran; Composites Research Laboratory (CRLab), Amirkabir University of Technology, Tehran, Iran.
² Department of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran. Electronic address: Atefeh.solouk@aut.ac.ir.
³ New Technologies Research Center (NTRC), Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran; Composites Research Laboratory (CRLab), Amirkabir University of Technology, Tehran, Iran. Electronic address: saeedss@aut.ac.ir.
⁴ Department of Textile Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran.
⁵ ENT and Head and Neck Research Center, Department of Otolaryngology, Head and Neck Surgery, The Five Senses Institute, Hazrat Rasoul Hospital, Iran University of Medical Sciences (IUMS), Tehran, Iran.
⁶ Department of Bioactive Products, Faculty of Chemistry, Adam Mickiewicz University Poznan, 61-614 Poznan, Poland.

PMID: 36774875
DOI: 10.1016/j.jcis.2023.01.139

Abstract

Nanocarrier-based drug delivery systems have been designed into various structures that can effectively prevent cancer progression and improve the therapeutic cancer index. However, most of these delivery systems are designed to be simple nanostructures with several limitations, including low stability and burst drug release features. A nano-in-nano delivery technique is explored to address the aforementioned concerns. Accordingly, this study investigated the release behavior of a novel nanoparticles-in-nanofibers delivery system composed of capsaicin-loaded alginate nanoparticles embedded in polycaprolactone-chitosan nanofiber mats. First, alginate nanoparticles were prepared with different concentrations of cationic gemini surfactant and using nanoemulsion templates. The optimized formulation of alginate nanoparticles was utilized for loading capsaicin and exhibited a diameter of 19.42 ± 1.8 nm and encapsulation efficiency of 98.7 % ± 0.6 %. Likewise, blend polycaprolactone-chitosan nanofibers were prepared with different blend ratios of their solutions (i.e., 100:0, 80:20, 60:40) by electrospinning method. After the characterization of electrospun mats, the optimal nanofibers were employed for embedding capsaicin-loaded alginate nanoparticles. Our findings revealed that embedding capsaicin-loaded alginate nanoparticles in polycaprolactone-chitosan nanofibers, prolonged capsaicin release from 120 h to more than 500 h. Furthermore, the results of in vitro analysis demonstrated that the designed nanoplatform could effectively inhibit the proliferation of MCF-7 human breast cells while being nontoxic to human dermal fibroblasts (HDF). Collectively, the prepared nanocomposite drug delivery platform might be promising for the long-term and controlled release of capsaicin for the prevention and treatment of cancer.

Keywords: Cationic gemini surfactant; Drug release regulation; Electrospinning; Nanoemulsion; Nanofibers; Nanostructure design; Specific cancer therapy.

MeSH terms

Alginates
Capsaicin
Chitosan* / chemistry
Drug Delivery Systems
Drug Liberation
Humans
Nanofibers* / chemistry
Nanoparticles* / chemistry

Substances

Chitosan
polycaprolactone
Capsaicin
Alginates