Polysaccharide of Atractylodes macrocephala Koidz(PAMK) protects against DEHP-induced apoptosis in grass carp hepatocytes

Zongyi Sun; Dejun Li; Haibin Wang; Yu Ding; Chun Zhang; Shuyue Wang; Xuejiao Wang; Bingxin Li; Danning Xu

doi:10.1016/j.fsi.2023.108587

Polysaccharide of Atractylodes macrocephala Koidz(PAMK) protects against DEHP-induced apoptosis in grass carp hepatocytes

Fish Shellfish Immunol. 2023 Mar:134:108587. doi: 10.1016/j.fsi.2023.108587. Epub 2023 Feb 10.

Authors

Zongyi Sun¹, Dejun Li², Haibin Wang², Yu Ding³, Chun Zhang³, Shuyue Wang³, Xuejiao Wang³, Bingxin Li⁴, Danning Xu⁵

Affiliations

¹ College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China.
² College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, China.
³ College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, China.
⁴ College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China. Electronic address: libingxin212@126.com.
⁵ College of Animal Science & Technology, Zhongkai University of Agriculture and Engineering, Guangzhou, 510225, China. Electronic address: xdanning212@126.com.

PMID: 36773714
DOI: 10.1016/j.fsi.2023.108587

Abstract

Di(2-ethylhexyl) phthalate (DEHP) is a new environmental pollutant, which is widely used in plastic additives. DEHP and its metabolites pollute surface water and threaten the survival of fish. In order to investigate the mechanism of DEHP-induced apoptosis on grass carp hepatocytes, we treated grass carp hepatocytes with DEHP, and selected Atractylodes macrocephala Koidz (PAMK) to study its inhibitory effect on DEHP. The results showed that after DEHP exposure, apoptosis related proteins expression were increased significantly, leading to hepatocytes apoptosis. Moreover, AO/EB staining and Hoechst staining also showed that the number of apoptotic cells increased after DEHP exposure. It should be noted that PAMK simultaneous treatment could alleviate apoptosis induced by DEHP. The innovation of this study is that the application of Chinese herbal medicine (PAMK) to antagonize the damage of DEHP in fish was investigated for the first time. This study indicated that traditional Chinese medicine can also be used in fish production to reduce the accumulation of food-derived drugs.

Keywords: Apoptosis; DEHP; Grass carp hepatocytes; PAMK.

MeSH terms

Animals
Apoptosis
Atractylodes*
Carps*
Diethylhexyl Phthalate*
Hepatocytes
Polysaccharides / pharmacology

Substances

Diethylhexyl Phthalate
Polysaccharides