Proteins participating in plant cell morphogenesis are often encoded by large gene families, in some cases comprising paralogs with variable (modular) domain organization, as in the case of the formin (FH2 protein) family of actin nucleators that can have also additional functions. Unravelling the phylogeny of such a complex gene family brings a number of specific challenges but may be crucial for predictions of protein function and for experimental design. Here we present an overview of our "cottage industry" semi-manual bioinformatic approach, based mostly, though not exclusively, on freely available software tools, which we used to obtain insight into the evolutionary history of plant FH2 proteins and some other components of the plant cell morphogenesis apparatus.
Keywords: Formins; Molecular phylogenetics; Multidomain proteins; Multiple sequence alignment; Phylogenetic tree construction; Splicing prediction; Transcriptome analysis.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.