Collisions of microtubules with membrane-associated structures containing myosin VIII were recently described, and these data suggested that such collisions can happen between microtubules and other membrane-associated proteins. Such collisions may contribute to a coordinated organization between microtubules and membrane-associated proteins especially in cases of low lateral diffusion rates of the protein. Coordinated organization of cortical cytoskeleton and membrane structures can have consequences on membrane compartmentalization and downstream signaling. Here we describe a way to analyze collisions of cortical microtubules and membrane-associated proteins by confocal microscopy. In addition, we describe a tool to measure and quantify these collisions.
Keywords: Collisions; Confocal microscopy; Live cell imaging; Membrane-associated proteins; Microtubules; Myosin VIII.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.