Actin: Static and Dynamic Studies

Huaqiang Ruan; Sha Zhang; Yi Zhang; Haiyun Ren

doi:10.1007/978-1-0716-2867-6_2

Actin: Static and Dynamic Studies

Methods Mol Biol. 2023:2604:13-24. doi: 10.1007/978-1-0716-2867-6_2.

Authors

Huaqiang Ruan¹, Sha Zhang¹, Yi Zhang², Haiyun Ren^{3

4}

Affiliations

¹ Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, Beijing, China.
² Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, Beijing, China. yi.zhang@bnu.edu.cn.
³ Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, Beijing, China. hren@bnu.edu.cn.
⁴ Center for Biological Science and Technology, Guangdong Zhuhai-Macao Joint Biotech Laboratory, Advanced Institute of Natural Science, Beijing Normal University, Zhuhai, China. hren@bnu.edu.cn.

PMID: 36773222
DOI: 10.1007/978-1-0716-2867-6_2

Abstract

The actin cytoskeleton is a highly dynamic network in plant cells, which is precisely regulated by numerous actin-binding proteins. Hence, characterizing the biochemical activities of actin-binding proteins is of great importance. Here we describe methods for determining the binding and bundling of microfilaments as well as methods for visualizing microfilaments using fluorescent phalloidin and single-molecule TIRF imaging.

Keywords: Actin; Actin-binding proteins; AtFH14; Microfilament binding and bundling assays; Single-molecule imaging; TIRF.

MeSH terms

Actin Cytoskeleton* / metabolism
Actins* / metabolism
Coloring Agents / metabolism
Microfilament Proteins / metabolism
Phalloidine / metabolism

Substances

Actins
Microfilament Proteins
Coloring Agents
Phalloidine