Medaka (Oryzias latipes), along with zebrafish (Danio rerio), is a useful experimental model fish. Here, we describe a simple method for generating medaka gene knockout strains using an automated microchip electrophoresis system. We also describe a method for targeted gene knockin using a plasmid carrying a sequence that does not cause off-target effects in medaka. Additionally, knockin method without plasmid cloning is described.
Keywords: CRISPR/Cas; Genome editing; Heteroduplex mobility assay; Medaka; Targeted integration; Targeted mutagenesis.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.