Generation of Knock-In Mouse by Genome Editing

Abstract

Knock-in mice are useful for evaluating endogenous gene expressions and functions in vivo. Instead of the conventional gene-targeting method using embryonic stem cells, an exogenous DNA sequence can be inserted into the target locus in the zygote using genome-editing technology. In this chapter, I describe the generation of epitope-tagged mice using engineered endonuclease and single-strand oligodeoxynucleotide through the mouse zygote as an example of how to generate a knock-in mouse by genome editing.

Keywords: Engineered endonuclease; Genome editing; Knock-in mice; Single-strand oligodeoxynucleotide (ssODN); Zygote.