Speculation of Sphingolipids in Capsanthin by Ultra-Performance Liquid Chromatography Coupled with Electrospray Ionization-Quadrupole-Time-of-Flight Mass Spectrometry

Molecules. 2023 Jan 19;28(3):1010. doi: 10.3390/molecules28031010.

Abstract

Sphingolipids are constituents of cellular membranes and play important roles in cells. As nutraceutical compounds in foods, sphingolipids have been proven to be critical for human health. Therefore, the sphingolipids content of capsanthin was established based on ultra-performance liquid chromatography coupled with electrospray ionization-quadrupole-time-of-flight mass spectrometry. A total number of 40 sphingolipids were successfully identified, including 20 Glucosylceramides and 20 Ceramides. The predominant GlcCers contain 4-hydroxy-8-sphingenine t18:1 (8) with different structures of α-OH fatty acids. For the Cers, the main long-chain bases are 4-hydroxy-8-sphingenine t18:1 (8) and 4-hydroxysphingenine (t18:0) with different structures of α-OH or α, β-di (OH) fatty acids.

Keywords: UPLC-Q-TOF HRMS/HRMS; capsanthin; ceramides; glucosylceramides.

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Liquid
  • Fatty Acids
  • Glucosylceramides / analysis
  • Humans
  • Spectrometry, Mass, Electrospray Ionization* / methods
  • Sphingolipids*

Substances

  • Sphingolipids
  • 4-hydroxy-8-sphingenine
  • capsanthin
  • Glucosylceramides
  • Fatty Acids

Grants and funding

This research was funded by Screening of plant active ingredients for improving intestinal mucosal barrier function of chicken and its underlying mechanism, grant number 2022YEE0111100.