Identification and Validation of Potentially Clinically Relevant CpG Regions within the Class 2 Tumor Suppressor Gene SFRP1 in Pancreatic Cancer

Maximilian Hauschulz; Sophia Villwock; Jennifer Kosinski; Florian Steib; Lara Rosaline Heij; Jan Bednarsch; Ruth Knüchel-Clarke; Edgar Dahl

doi:10.3390/cancers15030683

Identification and Validation of Potentially Clinically Relevant CpG Regions within the Class 2 Tumor Suppressor Gene SFRP1 in Pancreatic Cancer

Cancers (Basel). 2023 Jan 22;15(3):683. doi: 10.3390/cancers15030683.

Authors

Maximilian Hauschulz^{1

2}, Sophia Villwock^{1

2}, Jennifer Kosinski^{1

2}, Florian Steib^{1

2}, Lara Rosaline Heij^{2

3}, Jan Bednarsch^{2

3}, Ruth Knüchel-Clarke^{1

2}, Edgar Dahl^{1

2

4}

Affiliations

¹ Institute of Pathology, Medical Faculty, RWTH Aachen University, D-52074 Aachen, Germany.
² Center for Integrated Oncology Aachen Bonn Cologne Duesseldorf (CIO ABCD), D-52074 Aachen, Germany.
³ Department of Surgery and Transplantation, Medical Faculty, RWTH Aachen University, D-52074 Aachen, Germany.
⁴ RWTH centralized Biomaterial Bank (RWTH cBMB), Medical Faculty, RWTH Aachen University, D-52074 Aachen, Germany.

Abstract

In pancreatic cancer treatment, tumor stage-dependent chemotherapies are used to prolong overall survival. By measuring DNA promoter hypermethylation in the plasma of patients with stage IV pancreatic cancer, it was recently shown that promoter DNA methylation of the tumor suppressor gene SFRP1 has a high value for predicting failure of drug treatment with gemcitabine. In this study, we therefore aimed to identify as precisely as possible the region in the SFRP1 promoter that is frequently hypermethylated in pancreatic cancer tissue. First, we used the TCGA data set to define CpG-rich regions flanking the SFRP1 transcription start site that were significantly more methylated in pancreatic cancer compared to normal pancreatic acinar tissue. A core CpG island was identified that exhibited abundant tumor DNA methylation and anti-correlation of SFRP1 mRNA expression. To validate our in silico results, we performed bisulfide conversion followed by DNA pyrosequencing of 28 matched formalin-fixed, paraffin-embedded (FFPE) pancreatic cancer cases and six pancreatic cancer cell lines. A defined block of seven CpG sites within the core CpG island was identified, which confirmed our in silico results by showing significantly higher SFRP1 methylation in pancreatic cancer specimens than in normal pancreatic tissue. By selecting this core CpG island, we were able to determine a median overall survival benefit for the low SFRP1 methylation group compared to the high SFRP1 methylation group (702 versus 517 days, p = 0.01) in the TCGA pancreatic cancer cohort. We propose a compact pyrosequencing assay that can be used in the future to further investigate the prognostic value of SFRP1 promoter hypermethylation in predicting pancreatic cancer chemoresistance. Therefore, instead of DNA analysis from blood (liquid biopsy), DNA easily extractable from cancer tissue blocks (FFPE material) could be used.

Keywords: FFPE; SFRP1; SFRP1 promoter hypermethylation; chemotherapy; class 2 tumor suppressor genes; pancreatic cancer; pyrosequencing.

Grants and funding

331065168/Deutsche Forschungsgemeinschaft