Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing

Surya Prasad Adhikari; Astha Paudel; Anisha Sharma; Baruna Thapa; Neha Khanal; Nisha Shastri; Sourav Rai; Rameshwar Adhikari

doi:10.1155/2023/8541621

Development of Decellularized Fish Skin Scaffold Decorated with Biosynthesized Silver Nanoparticles for Accelerated Burn Wound Healing

Int J Biomater. 2023 Jan 31:2023:8541621. doi: 10.1155/2023/8541621. eCollection 2023.

Authors

Surya Prasad Adhikari^{1

2}, Astha Paudel², Anisha Sharma², Baruna Thapa², Neha Khanal², Nisha Shastri², Sourav Rai², Rameshwar Adhikari³

Affiliations

¹ Department of Mechanical and Aerospace Engineering, Institute of Engineering, Pulchowk Campus, Tribhuvan University, Lalitpur, Nepal.
² College of Biomedical Engineering and Applied Sciences, Purbanchal University, Lalitpur, Nepal.
³ Centre Department of Chemistry, Tribhuvan University, Kirtipur, Nepal.

Abstract

In this study, decellularized fish skin (DFS) scaffold decorated with silver nanoparticles was prepared for accelerating burn wound healing. The silver nanoparticles (AgNPs) synthesized by the green and facile method using Aloe vera leaf at different incubating times were characterized by using X-ray Diffraction (XRD), Fourier Transform Infrared (FT-IR) Spectroscopy, and Ultraviolet-Visible Spectroscopy (UV-Vis spectroscopy). The different characterizations confirmed that the sizes of AgNPs prepared by incubating for 6 hours and 12 hours were 29.1 nm and 35.2 nm, respectively. After that, the different concentrations of the smallest AgNPs were used to dope the DFS scaffold to determine the cell viability. Additionally, an agar well diffusion method was used to screen for antimicrobial activity. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were used to correlate the concentration of AgNPs with its bactericidal effect which was seen from 50 μg/ml. Then, the toxicity with human cells was investigated using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay with no significant cell viability from the concentration of 50 μg/ml to 200 μg/ml compared to the cocultured and commercial treatments.