Visualizing intracellular target antigens in live cells

Hiroshi Ueda; Yancen Dai; Farid Ghadessy

doi:10.1016/j.tcb.2023.01.004

Visualizing intracellular target antigens in live cells

Trends Cell Biol. 2023 Apr;33(4):277-279. doi: 10.1016/j.tcb.2023.01.004. Epub 2023 Feb 7.

Authors

Hiroshi Ueda¹, Yancen Dai², Farid Ghadessy³

Affiliations

¹ Graduate School of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta-cho, Yokohama, Kanagawa 226-8503, Japan.
² Laboratory for Chemistry and Life Science, Institute of Innovative Research, Tokyo Institute of Technology, Nagatsuta-cho, Yokohama, Kanagawa 226-8503, Japan.
³ Disease Intervention Technology Laboratory, Institute of Molecular and Cellular Biology, Agency for Science Technology and Research (A*STAR), Singapore, Singapore. Electronic address: fghadessy@imcb.a-star.edu.sg.

PMID: 36759281
DOI: 10.1016/j.tcb.2023.01.004

Abstract

In order to further visualize intracellular dynamics, precise imaging of endogenous proteins in live cells was performed using an antigen-binding fragment (Fab)-based Quenchbody (Q-body). The transfected Q-body probe showed an antigen-dependent fluorescence response, enabling the clear visualization and sorting of cells expressing p53, a tumor suppressor biomarker.

Keywords: cancer diagnostics; cellular immunofluorescence; live-cell imaging; p53; quenchbody.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers, Tumor
Fluorescence
Fluorescent Dyes* / metabolism
Humans
Proteins*

Substances

Fluorescent Dyes
Proteins
Biomarkers, Tumor