Biolayer interferometry is insufficiently sensitive for direct measurement of IgM quantity and avidity in Atlantic salmon (Salmo salar) serum

Chloe J English; Angus Li; Andrew C Barnes

doi:10.1016/j.fsi.2023.108603

Biolayer interferometry is insufficiently sensitive for direct measurement of IgM quantity and avidity in Atlantic salmon (Salmo salar) serum

Fish Shellfish Immunol. 2023 Mar:134:108603. doi: 10.1016/j.fsi.2023.108603. Epub 2023 Feb 8.

Authors

Chloe J English¹, Angus Li², Andrew C Barnes²

Affiliations

¹ The University of Queensland, School of Biological Sciences, Brisbane, QLD, 4072, Australia. Electronic address: chloe.english@uq.edu.au.
² The University of Queensland, School of Biological Sciences, Brisbane, QLD, 4072, Australia.

PMID: 36758657
DOI: 10.1016/j.fsi.2023.108603

Abstract

Quantification of specific antibodies underpins the assessment of adaptive immunity in response to vaccination or infection and is performed by enzyme-linked immunosorbent assay (ELISA). A biolayer interferometry (BLI) assay was recently developed that simultaneously quantifies IgM antibodies and their avidity in giant grouper (Epinephelus lanceolatus) sera and proved to be a robust, repeatable and more high-throughput alternative to ELISA [1]. Here we attempted to optimise a similar single-step BLI assay using an Octet HTX instrument to quantify IgM specific to the hapten 2,4-dinitrophenol (DNP) in serum from Atlantic salmon (Salmo salar) primed and boosted with DNP conjugated to keyhole limpet hemocyanin.

Keywords: Antibody; Biolayer interferometry; ELISA; Fish IgM; Octet HTX.

MeSH terms

Animals
Enzyme-Linked Immunosorbent Assay
Immunoglobulin M
Interferometry
Salmo salar*
Vaccination

Substances

Immunoglobulin M