Genome-based risk assessment for foodborne Salmonella enterica from food animals in China: A One Health perspective

Biao Tang; Abubakar Siddique; Chenhao Jia; Abdelaziz Ed-Dra; Jing Wu; Hui Lin; Min Yue

doi:10.1016/j.ijfoodmicro.2023.110120

Genome-based risk assessment for foodborne Salmonella enterica from food animals in China: A One Health perspective

Int J Food Microbiol. 2023 Apr 2:390:110120. doi: 10.1016/j.ijfoodmicro.2023.110120. Epub 2023 Feb 4.

Authors

Biao Tang¹, Abubakar Siddique², Chenhao Jia³, Abdelaziz Ed-Dra⁴, Jing Wu¹, Hui Lin¹, Min Yue⁵

Affiliations

¹ State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products & Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang, China.
² Hainan Institute of Zhejiang University, Sanya 572025, China; Atta Ur Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Islamabad 44000, Pakistan.
³ Hainan Institute of Zhejiang University, Sanya 572025, China; Department of Veterinary Medicine, Institute of Preventive Veterinary Sciences, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China.
⁴ Hainan Institute of Zhejiang University, Sanya 572025, China.
⁵ Hainan Institute of Zhejiang University, Sanya 572025, China; Department of Veterinary Medicine, Institute of Preventive Veterinary Sciences, College of Animal Sciences, Zhejiang University, Hangzhou 310058, China; State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, National Medical Center for Infectious Diseases, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China; Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine, Hangzhou, China. Electronic address: myue@zju.edu.cn.

PMID: 36758302
DOI: 10.1016/j.ijfoodmicro.2023.110120

Abstract

Salmonella is one of the most common causes of foodborne bacterial disease. Animal-borne foods are considered the primary sources of Salmonella transmission to humans. However, genomic assessment of antimicrobial resistance (AMR) and virulence of Salmonella based on One Health approach remains obscure in China. For this reason, we analyzed the whole genome sequencing data of 134 Salmonella isolates recovered from different animal and meat samples in China. The 134 Salmonella were isolated from 2819 samples (4.75 %) representing various sources (pig, chicken, duck, goose, and meat) from five Chinese provinces (Zhejiang, Guangdong, Jiangxi, Hunan, and Qinghai). AMR was evaluated by the broth dilution method using 13 different antimicrobial agents, and results showed that 85.82 % (115/134) of isolates were resistant to three or more antimicrobial classes and were considered multidrug-resistant (MDR). Twelve sequence types (STs) were detected, with a dominance of ST469 (29.85 %, 40/134). The prediction of virulence genes showed the detection of cdtB gene encoding typhoid toxins in one isolate of S. Muenster recovered from chicken, while virulence genes associated with type III secretion systems were detected in all isolates. Furthermore, plasmid-type prediction showed the abundance of IncFII(S) (13/134; 9.7 %) and IncFIB(S) (12/134; 8.95 %) in the studied isolates. Together, this study demonstrated the ability to use whole-genome sequencing (WGS) as a cost-effective method to provide comprehensive knowledge about foodborne Salmonella isolates in One Health surveillance approach.

Keywords: Antimicrobial resistance gene; Food animals; One health; Salmonella; Whole genome sequencing.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology
Drug Resistance, Multiple, Bacterial / genetics
Foodborne Diseases* / microbiology
Humans
Microbial Sensitivity Tests
One Health*
Salmonella
Salmonella enterica*
Swine

Substances

Anti-Bacterial Agents