In vitro magnetic hyperthermia properties of angle-shaped superparamagnetic iron oxide nanoparticles synthesized by a bromide-assisted polyol method

Hoonsub Kim; Pyung Won Im; Chaedong Lee; Hwichan Hong; Wooseung Lee; Changhyuk Koo; Sang Yoon Park; Hyung-Jun Im; Sun Ha Paek; Yuanzhe Piao

doi:10.1039/d2ra07190h

In vitro magnetic hyperthermia properties of angle-shaped superparamagnetic iron oxide nanoparticles synthesized by a bromide-assisted polyol method

RSC Adv. 2023 Jan 19;13(5):2803-2810. doi: 10.1039/d2ra07190h. eCollection 2023 Jan 18.

Authors

Hoonsub Kim¹, Pyung Won Im², Chaedong Lee¹, Hwichan Hong¹, Wooseung Lee¹, Changhyuk Koo¹, Sang Yoon Park³, Hyung-Jun Im^{1

2

4}, Sun Ha Paek^{2

4

5}, Yuanzhe Piao^{1

3}

Affiliations

¹ Department of Applied Bioengineering, Graduate School of Convergence Science and Technology, Seoul National University Suwon-si Gyeonggi-do 16229 Republic of Korea iiihjjj@snu.ac.kr.
² Cancer Research Institute, Seoul National University Seoul 03080 Republic of Korea paeksh@snu.ac.kr.
³ Advanced Institutes of Convergence Technology Suwon-si Gyeonggi-do 16229 South Korea parkat9@snu.ac.kr +82 31 888 9141.
⁴ Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University Seoul 08826 Republic of Korea.
⁵ Department of Neurosurgery, Hypoxia/Ischemia Disease Institute, Seoul National University Seoul 03080 Republic of Korea.

Abstract

Currently, research on superparamagnetic iron oxide nanoparticles (SPIONs) for magnetic hyperthermia applications is steadily increasing. In this work, SPIONs were synthesized by the bromide-assisted polyol method and angle-shaped SPIONs were successfully generated with the optimized concentration of bromide. The influence of bromide concentration on the shape of the generated SPIONs as well as the heating characteristics under an alternating magnetic field (AMF) was thoroughly investigated. At a concentration of 20 mg mL^-1 of the angle-shaped SPIONs, the highest temperature curve up to 23 °C was observed under AMF with 140 Oe and 100 kHz for 10 min. With the biotoxicity assay, no significant cytotoxicity was observed in the normal fibroblast of HFB-141103 as well as tumor cells of U87MG and FSall treated with the angle-shaped SPIONs at a concentration below 100 μg mL^-1. However, significantly decreased cellular viability was observed in tumor cells of U87MG and FSall treated with 100 μg mL^-1 of the angle-shaped SPIONs under AMF with 140 Oe and 100 kHz. Based on these results, it is thought that the angle-shaped SPIONs synthesized by the bromide-assisted polyol method will provide highly efficient magnetic hyperthermia therapy for cancers under biologically safe AMF with 140 Oe and 100 kHz.