3D genome alterations and editing in pathology

Eugenia A Tiukacheva; Sergey V Ulianov; Anna Karpukhina; Sergey V Razin; Yegor Vassetzky

doi:10.1016/j.ymthe.2023.02.005

3D genome alterations and editing in pathology

Mol Ther. 2023 Apr 5;31(4):924-933. doi: 10.1016/j.ymthe.2023.02.005. Epub 2023 Feb 8.

Authors

Eugenia A Tiukacheva¹, Sergey V Ulianov², Anna Karpukhina³, Sergey V Razin², Yegor Vassetzky⁴

Affiliations

¹ CNRS UMR9018, Institut Gustave Roussy, 94805 Villejuif, France; Institute of Gene Biology, Moscow 119334, Russia; Moscow Institute of Physics and Technology, Moscow 141700, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow 119991, Russia; Koltzov Institute of Developmental Biology, Moscow 119334, Russia.
² Institute of Gene Biology, Moscow 119334, Russia; Faculty of Biology, Lomonosov Moscow State University, Moscow 119991, Russia.
³ CNRS UMR9018, Institut Gustave Roussy, 94805 Villejuif, France; Koltzov Institute of Developmental Biology, Moscow 119334, Russia.
⁴ CNRS UMR9018, Institut Gustave Roussy, 94805 Villejuif, France; Koltzov Institute of Developmental Biology, Moscow 119334, Russia. Electronic address: yegor.vassetzky@cnrs.fr.

Abstract

The human genome is folded into a multi-level 3D structure that controls many nuclear functions including gene expression. Recently, alterations in 3D genome organization were associated with several genetic diseases and cancer. As a consequence, experimental approaches are now being developed to modify the global 3D genome organization and that of specific loci. Here, we discuss emerging experimental approaches of 3D genome editing that may prove useful in biomedicine.

Keywords: 3D genome; cancer; epigenome editing; genetic diseases; therapy.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

CRISPR-Cas Systems
Cell Nucleus
Gene Editing*
Genome, Human
Humans
Neoplasms* / genetics
Neoplasms* / therapy