Anti-obesity mechanism of Ganpu tea revealed by microbiome, metabolome and transcriptome analyses

Yanfang Liao; Chen Wang; Zhipeng Gao; Zhaoping Pan; Mingfang Peng; Jie Ma; Yujiao Qian; Jiajing Guo; Fuhua Fu

doi:10.1016/j.foodchem.2022.135048

Anti-obesity mechanism of Ganpu tea revealed by microbiome, metabolome and transcriptome analyses

Food Chem. 2023 Jun 30:412:135048. doi: 10.1016/j.foodchem.2022.135048. Epub 2022 Dec 22.

Authors

Yanfang Liao¹, Chen Wang¹, Zhipeng Gao², Zhaoping Pan³, Mingfang Peng¹, Jie Ma², Yujiao Qian¹, Jiajing Guo⁴, Fuhua Fu⁵

Affiliations

¹ Longping Branch, College of Biology, Hunan University, Changsha 410125, China; International Joint Lab on Fruits &Vegetables Processing, Quality and Safety, Hunan Key Lab of Fruits &Vegetables Storage, Processing, Quality and Safety, Hunan Agriculture Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
² College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China.
³ International Joint Lab on Fruits &Vegetables Processing, Quality and Safety, Hunan Key Lab of Fruits &Vegetables Storage, Processing, Quality and Safety, Hunan Agriculture Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China.
⁴ International Joint Lab on Fruits &Vegetables Processing, Quality and Safety, Hunan Key Lab of Fruits &Vegetables Storage, Processing, Quality and Safety, Hunan Agriculture Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China. Electronic address: guojiajing1986@163.com.
⁵ Longping Branch, College of Biology, Hunan University, Changsha 410125, China; International Joint Lab on Fruits &Vegetables Processing, Quality and Safety, Hunan Key Lab of Fruits &Vegetables Storage, Processing, Quality and Safety, Hunan Agriculture Product Processing Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China. Electronic address: fhfu686@163.com.

PMID: 36753939
DOI: 10.1016/j.foodchem.2022.135048

Abstract

In this study, the anti-obesity mechanism of Ganpu tea (GPT) from the perspectives of microbiome, metabolome and transcriptome was investigated. GPT significantly reduced the high-fat-diet (HFD)-induced levels of inflammatory cytokines and the expansion of lipid droplets and white adipose tissue. GPT also improved HFD-induced gut microbiome imbalance by significantly reducing the proportion of Firmicutes to Bacteroidota. Metabolomic data showed that HFD-induced metabolic disorder was regulated by GPT and probably characterised by being related to 4-aminobutyraldehyde and 5-acetylamino-6-amino-3-methyluracil. Transcriptome showed that the improvement of obesity was mainly related to the IL-17 signaling pathway and the metabolism of xenobiotics by cytochrome P450. Spearman's correlation analysis indicated that gut microbiota were significantly correlated with inflammatory factors, genes and metabolites. Metabolome-transcriptome analysis showed that GPT reversed obesity mainly through the carbohydrate metabolism, amino acid metabolism and lipid metabolism.Collectively, GPT may be used as a health drink to prevent or treat obesity.

Keywords: Ganpu tea; Metabolome; Microbiome; Obesity; Transcriptome.

MeSH terms

Animals
Diet, High-Fat
Gastrointestinal Microbiome* / genetics
Gene Expression Profiling
Lipid Metabolism / genetics
Metabolome
Mice
Mice, Inbred C57BL
Obesity* / drug therapy
Obesity* / genetics
Obesity* / metabolism
Tea / chemistry

Substances

Tea