Structure of the lipopolysaccharide O-antigen of Serratia spp. strains 10.1WK and 1XS plant endophytes isolated from O. biennis and L. corniculatus

Anita Swatek; Zbigniew Kaczyński; Katarzyna Kasperkiewicz; Małgorzata Pawlik; Iwona Komaniecka; Adam Choma

doi:10.1016/j.carres.2023.108760

Structure of the lipopolysaccharide O-antigen of Serratia spp. strains 10.1WK and 1XS plant endophytes isolated from O. biennis and L. corniculatus

Carbohydr Res. 2023 Feb:524:108760. doi: 10.1016/j.carres.2023.108760. Epub 2023 Jan 25.

Authors

Anita Swatek¹, Zbigniew Kaczyński², Katarzyna Kasperkiewicz³, Małgorzata Pawlik³, Iwona Komaniecka¹, Adam Choma⁴

Affiliations

¹ Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland.
² Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, 80-308, Gdansk, Poland.
³ Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032, Katowice, Poland.
⁴ Department of Genetics and Microbiology, Institute of Biological Sciences, Maria Curie-Sklodowska University, Akademicka 19, 20-033, Lublin, Poland. Electronic address: adam.choma@mail.umcs.pl.

PMID: 36753890
DOI: 10.1016/j.carres.2023.108760

Abstract

O-specific polysaccharides (O-PSs) isolated from lipopolysaccharides of Serratia spp., strains 10.1WK and 1XS, which are endophytic bacteria of Oenothera biennis (common evening-primrose) and Lotus corniculatus (bird's-foot trefoil), plants growing on a petroleum hydrocarbon polluted site in the Silesia region, were investigated. The high-molecular-weight O-PS fractions liberated from lipopolysaccharides by mild acid hydrolysis were studied using chemical methods, MALDI-TOF mass spectrometry, and a set of 1D and 2D NMR spectroscopy techniques. It was found that both O-specific polysaccharides were built of an identical trisaccharide repeating unit composed of d-Rhap and d-Manp residues. The following structure of the O-PSs of Serratia spp. strains 10.1WK and 1XS was established: →4)-α-d-Rhap-(1 → 3)-β-d-Manp-(1 → 4)-β-d-Rhap-(1→.

Keywords: Lipopolysaccharide; MALDI-TOF; NMR; O-specific polysaccharide; Plant endophytes; Serratia spp.

MeSH terms

Endophytes
Lipopolysaccharides* / chemistry
Lotus / microbiology
Magnetic Resonance Spectroscopy / methods
O Antigens* / chemistry
Oenothera / microbiology
Serratia* / chemistry

Substances

Lipopolysaccharides
O Antigens