Manufacture of extracellular vesicles derived from mesenchymal stromal cells

Paula M Pincela Lins; Elke Pirlet; Michal Szymonik; Annelies Bronckaers; Inge Nelissen

doi:10.1016/j.tibtech.2023.01.003

Manufacture of extracellular vesicles derived from mesenchymal stromal cells

Trends Biotechnol. 2023 Jul;41(7):965-981. doi: 10.1016/j.tibtech.2023.01.003. Epub 2023 Feb 6.

Authors

Paula M Pincela Lins¹, Elke Pirlet², Michal Szymonik³, Annelies Bronckaers⁴, Inge Nelissen⁵

Affiliations

¹ Hasselt University, Faculty of Medicine and Life Sciences, Biomedical Research Institute (BIOMED), Agoralaan, 3590 Diepenbeek, Belgium; Flemish Institute for Technological Research (VITO), Health Department, Boeretang, 2400 Mol, Belgium.
² Hasselt University, Faculty of Medicine and Life Sciences, Biomedical Research Institute (BIOMED), Agoralaan, 3590 Diepenbeek, Belgium.
³ Flemish Institute for Technological Research (VITO), Health Department, Boeretang, 2400 Mol, Belgium.
⁴ Hasselt University, Faculty of Medicine and Life Sciences, Biomedical Research Institute (BIOMED), Agoralaan, 3590 Diepenbeek, Belgium. Electronic address: annelies.bronckaers@uhasselt.be.
⁵ Flemish Institute for Technological Research (VITO), Health Department, Boeretang, 2400 Mol, Belgium. Electronic address: inge.nelissen@vito.be.

PMID: 36750391
DOI: 10.1016/j.tibtech.2023.01.003

Abstract

Mesenchymal stromal cells (MSCs) are a promising therapy for various diseases ranging from ischemic stroke to wound healing and cancer. Their therapeutic effects are mainly mediated by secretome-derived paracrine factors, with extracellular vesicles (EVs) proven to play a key role. This has led to promising research on the potential of MSC-EVs as regenerative, off-the-shelf therapeutic agents. However, the translation of MSC-EVs into the clinic is hampered by the poor scalability of their production. Recently, new advanced methods have been developed to upscale MSC cultivation and EV production yields, ranging from new cell culture devices to priming procedures. This review gives an overview of these innovative strategies for manufacturing MSC-EVs.

Keywords: bioreactor; extracellular vesicles; mesenchymal stromal cells; upscaled manufacturing.

Publication types

Review

MeSH terms

Cell Culture Techniques
Extracellular Vesicles*
Mesenchymal Stem Cells*
Wound Healing