Flow cytometry data on the effect of dimethylacetamide and N-methylacetamide used at different concentrations on the quality of cryopreserved chicken semen

Manuela Madeddu; Luisa Zaniboni; Stefano Paolo Marelli; Stefano Comazzi; Silvia Cerolini

doi:10.1016/j.dib.2023.108916

Flow cytometry data on the effect of dimethylacetamide and N-methylacetamide used at different concentrations on the quality of cryopreserved chicken semen

Data Brief. 2023 Jan 19:47:108916. doi: 10.1016/j.dib.2023.108916. eCollection 2023 Apr.

Authors

Manuela Madeddu¹, Luisa Zaniboni¹, Stefano Paolo Marelli¹, Stefano Comazzi¹, Silvia Cerolini¹

Affiliation

¹ Department of Veterinary Medicine and Animal Science, University of Milan, via dell'Università 6, Lodi 26900, Italy.

Abstract

This article includes supporting data regarding the research article entitled "Concentration dependent effect of dimethylacetamide and N-methylacetamide on the quality and fertility of cryopreserved chicken semen" (Zaniboni et al., 2022). The effect of two permeant-cryoprotectants (CPA), dimethylacetamide (DMA) and N-methylacetamide (NMA) used at different concentrations (0, 2, 4 and 6%) on the quality of post thaw rooster semen was assessed. Ejaculates were processed according to 7 treatments: Lake pre-freezing+0.1 M trehalose (LPF-T) (control treatment), LPF-T+2% DMA (DMA2), LPF-T+4% DMA (DMA4), LPF-T+6% DMA (DMA6), LPF-T+2% NMA (NMA2), LPF-T+4% NMA (NMA4), LPF-T+6% NMA (NMA6). Sperm acrosome integrity and mitochondrial activity were investigated in frozen-thawed semen with the use of the flow cytometry technique. Only the mitochondrial activity was significantly affected by the different cryoprotectant concentrations.

Keywords: Acrosome integrity; Cryoprotectant; Mitochondrial activity; Poultry semen; Sperm cryopreservation.