Transcriptional activation of CSTB gene expression by transcription factor Sp3

Weiyi Zhu; Xiangjun Dong; Shuyue Luo; Shipeng Guo; Weihui Zhou; Weihong Song

doi:10.1016/j.bbrc.2023.01.087

Transcriptional activation of CSTB gene expression by transcription factor Sp3

Biochem Biophys Res Commun. 2023 Mar 15:649:71-78. doi: 10.1016/j.bbrc.2023.01.087. Epub 2023 Jan 30.

Authors

Weiyi Zhu¹, Xiangjun Dong¹, Shuyue Luo¹, Shipeng Guo¹, Weihui Zhou², Weihong Song³

Affiliations

¹ Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China.
² Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China. Electronic address: zwh@hospital.cqmu.edu.cn.
³ Chongqing Key Laboratory of Translational Medical Research in Cognitive Development and Learning and Memory Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Children's Hospital of Chongqing Medical University, Chongqing, China; Institute of Aging, Key Laboratory of Alzheimer's Disease of Zhejiang Province, Zhejiang Provincial Clinical Research Center for Mental Disorders, School of Mental Health and Kangning Hospital, The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, 325000, China; Oujiang Laboratory Zhejiang Lab for Regenerative Medicine, Vision and Brain Health, Wenzhou, Zhejiang, 325001, China. Electronic address: weihong@wmu.edu.cn.

PMID: 36745972
DOI: 10.1016/j.bbrc.2023.01.087

Abstract

CSTB has been reported to be associated with the pathogenesis of many malignant tumors, especially hepatocellular carcinoma (HCC). However, how the expression of this gene is regulated is largely unknown. We initially cloned and analyzed the promoter region of the CSTB gene by luciferase assay and the Sp3 binding site (CCCCGCCCCGCG) was found in it. The results of electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) experiments verified that the transcription factor, Sp3 could bind to the " CCCCGCCCCGCG ″ site of the CSTB gene promoter. We showed that the overexpression of Sp3 significantly increased the endogenous mRNA and protein expression levels of CSTB, whereas knockdown of Sp3 decreased the mRNA and protein expression levels according to quantitative real-time PCR (qRT‒PCR) and western blotting. In conclusion, CSTB gene expression is closely regulated by transcription factor Sp3, which may be a potential mechanism for the dysregulation of CSTB expression in HCC.

Keywords: CSTB; Hepatocellular carcinoma; Sp3; Transcriptional regulation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma, Hepatocellular* / genetics
Gene Expression
Humans
Liver Neoplasms* / genetics
RNA, Messenger / genetics
RNA, Messenger / metabolism
Sp1 Transcription Factor / genetics
Sp1 Transcription Factor / metabolism
Sp3 Transcription Factor / genetics
Transcriptional Activation

Substances

Sp3 Transcription Factor
RNA, Messenger
Sp1 Transcription Factor