An odyssey from laboratory to field ? - Portable tNGS system for TB diagnosis in programmatic setting

Radha Gopalaswamy; Bhargavi Subramanian; Padmapriyadarsini Chandrasekaran; Siva Kumar Shanmugam

doi:10.1016/j.ijtb.2022.04.004

An odyssey from laboratory to field ? - Portable tNGS system for TB diagnosis in programmatic setting

Indian J Tuberc. 2023 Jan;70(1):1-3. doi: 10.1016/j.ijtb.2022.04.004. Epub 2022 Apr 29.

Authors

Radha Gopalaswamy¹, Bhargavi Subramanian², Padmapriyadarsini Chandrasekaran¹, Siva Kumar Shanmugam³

Affiliations

¹ ICMR-National Institute for Research in Tuberculosis, Chennai, India.
² SRM Institute of Science and Technology, India.
³ ICMR-National Institute for Research in Tuberculosis, Chennai, India. Electronic address: shanmugamsiva@nirt.res.in.

PMID: 36740303
DOI: 10.1016/j.ijtb.2022.04.004

Abstract

In spite of the elaborate diagnostic modalities available in India, there are certain shortcomings which will affect patient management. In order to address the gaps, NTEP offers scope for whole genome sequencing at few of its reference laboratories. Next generation sequencing comprising of whole genome sequencing (WGS) and targeted next generation sequencing (tNGS) are upcoming fields in TB diagnosis In a programmatic setting, tNGS offers great promise for smear positive or NAAT positive samples to be used with a Minion platform in a field setting beyond just the National reference laboratories. Once materialised, the tNGS would offer personalised patient management as well as help in public health by identification of outbreaks, transmission chain monitoring and drug resistance surveillance.

Keywords: Drug resistance; Mycobacterium tuberculosis; Nanopore sequencing; tNGS (Targetted next generation sequencing).

Publication types

Editorial

MeSH terms

Drug Resistance, Multiple, Bacterial / genetics
High-Throughput Nucleotide Sequencing
Humans
Laboratories
Mycobacterium tuberculosis* / genetics
Tuberculosis* / diagnosis
Whole Genome Sequencing