Analysis of sheep peripheral blood mononuclear cells in response to Echinococcus granulosus microRNA-71 overexpression

Yating Li; Lujun Yan; Duojie Ci; Rui Li; Wanjing Li; Tianqi Xia; Hengzhi Shi; Mazhar Ayaz; Yadong Zheng; Pu Wang

doi:10.1016/j.molbiopara.2023.111556

Analysis of sheep peripheral blood mononuclear cells in response to Echinococcus granulosus microRNA-71 overexpression

Mol Biochem Parasitol. 2023 Jun:254:111556. doi: 10.1016/j.molbiopara.2023.111556. Epub 2023 Feb 2.

Authors

Yating Li¹, Lujun Yan¹, Duojie Ci², Rui Li¹, Wanjing Li¹, Tianqi Xia¹, Hengzhi Shi¹, Mazhar Ayaz³, Yadong Zheng⁴, Pu Wang⁵

Affiliations

¹ Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, China.
² NHC Key Laboratory of Echinococcosis Prevention and Control, Tibet Center for Disease Control and Prevention, Lhasa 850000, Tibet Autonomous Region, China.
³ Cholistan University of Veterinary and Animal Sciences, Bahawalpur 73000, Pakistan.
⁴ Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, China. Electronic address: zhengyadong@zafu.edu.cn.
⁵ Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Laboratory for Animal Health Inspection & Internet Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics, College of Animal Science and Technology & College of Veterinary Medicine of Zhejiang A&F University, Hangzhou 311300, China. Electronic address: wangpu110@zafu.edu.cn.

PMID: 36739092
DOI: 10.1016/j.molbiopara.2023.111556

Abstract

Cyst echinococcosis, caused by Echinococcus granulosus, remains a zoonotic disease posing a great threat to public health and meat production industry. Sheep infected with E. granulosus show relatively high abundance of egr-miR-71 in the sera, but its role is unknown. Using bioinformatics and cell migration and Transwell assays, we comparatively analyzed the proteomes and cell invasion of sheep PBMCs in response to egr-miR-71 overexpression. The results showed that the egr-miR-71 induced a total of 157 proteins being differentially expressed and mainly involved in immune responses. In sheep PBMCs, egr-miRNA-71 overexpression induced significant downregulation of macrophage migration inhibitory factor (MIF) and accordingly promoted cell migration and invasion compared with the control. The results will provide a clue for further investigation of a role of circulating egr-miR-71 in immune responses during E. granulosus infection.

Keywords: Echinococcus granulosus; MIF; Sheep PBMC; egr-miR-71.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Echinococcosis*
Echinococcus granulosus* / genetics
Leukocytes, Mononuclear
MicroRNAs* / genetics
Sheep
Sheep Diseases*
Zoonoses

Substances

MicroRNAs