Auxenochlorella pyrenoidosa extract supplementation replacing fetal bovine serum for Carassius auratus muscle cell culture under low-serum conditions

Abstract

Cultured meat production requires large-scale cell proliferation in vitro with the supplementation of necessary media especially serum. This study investigated the capacity of Auxenochlorella pyrenoidosa extract (APE) to replace fetal bovine serum (FBS) for cell culture under low-serum conditions using Carassius auratus muscle (CAM) cells. Supplementation with APE and 5% FBS in the culture media significantly promoted the proliferation of CAM cells and increased the expression of MyoD in cells compared to that with 5% FBS through cell counting kit-8 and immunofluorescence staining assay. In addition, CAM cells in the media containing 5% FBS and APE could be continually cultured for 4 passages, and the cell number was 1.58 times higher than the counterpart without APE in long-term culture. Moreover, supplementation with APE realized large-scale culture on microcarriers under low-serum conditions, and more adherent cells were observed on microcarriers in 2% FBS supplemented with APE, compared with those in 2% FBS and 10% FBS without APE. These findings highlighted a potentially promising application of APE in muscle cell culture under low-serum conditions for cultured meat production.

Keywords: Auxenochlorella pyrenoidosa; Cultured meat; Muscle cell; Proliferation; Scale-up culture; Serum.