Dentin disinfection and adhesive bond strength using modified photoactivated carbon nanoparticles

Mashael Binhasan; Hanan Alsunbul; Mohammad Aljanakh; Tariq Abduljabbar; Fahim Vohra

doi:10.1016/j.pdpdt.2023.103313

Dentin disinfection and adhesive bond strength using modified photoactivated carbon nanoparticles

Photodiagnosis Photodyn Ther. 2023 Jun:42:103313. doi: 10.1016/j.pdpdt.2023.103313. Epub 2023 Feb 2.

Authors

Mashael Binhasan¹, Hanan Alsunbul², Mohammad Aljanakh³, Tariq Abduljabbar⁴, Fahim Vohra⁴

Affiliations

¹ Department of Restorative Dentistry, Division of Operative Dentistry, College of Dentistry, King Saud University, Saudi Arabia. Electronic address: mbinhasan@ksu.edu.sa.
² Department of Restorative Dentistry, College of Dentistry, King Saud University, Riyadh, Saudi Arabia.
³ Department of Restorative Dentistry, College of Dentistry, University of Ha'il, Hail 55473, Saudi Arabia.
⁴ Department of Prosthetic Dental Science, College of Dentistry, King Saud University, P. O. Box 60169, Riyadh 11545, Saudi Arabia.

PMID: 36736549
DOI: 10.1016/j.pdpdt.2023.103313

Abstract

Aims: The present study aimed to investigate the bond integrity and disinfection efficacy of Methylene blue(MB) alone, MB-PDT (Photodynamic therapy), MB@ carbon nanoparticles (CP)-PDT, and Cr, Cr: YSGG (ECL) against lactobacilli in Caries-affected dentin (CAD) MATERIAL AND METHODS: Methods consisted of Shear bond strength (SBS), Scanning electron microscopy (SEM), energy dispersive X-ray (EDX), methods of disinfection, and failure analysis. CAD samples were prepared and biofilm formed on the specimens randomly allocated into five groups based on disinfection. Group 1: CHX; Group 2: MB; Group 3: MB-PDT: group 4: MB@CP-PDT and group 5: ECL. After disinfection Colony forming units were measured and specimens were restored and positioned under a universal testing machine (UTM). Failure analysis was performed using a stereomicroscope. The difference in survival rate was assessed using the Kruskal-Walis test. Mean and standard deviation for bond values after different methods of disinfection was evaluated using analysis of variance (ANOVA) and Post Hoc Tukey. The significance level was p<0.05 RESULTS: Morphological analysis revealed that CPs under SEM are flat discs with edged irregular shapes. EDX analyses show a spike indicating carbon particles by more than 95%. MB@CP-PDT displayed the highest reduction in lactobacillus levels in comparison to the other disinfection methods. The highest SBS was exhibited by the CAD sample disinfected with ECL. The lowest SBS values in CAD specimens after cavity cleansing with MB alone. The predominant failure type in CAD disinfected with MB alone, ECL CHX, MB-PDT, and MB@CP-PDT was adhesive.

Conclusion: The use of MB@CP-PDT showed high antibacterial potency against lactobacillus but demonstrated bond values similar to CHX. Use of Er, Cr: YSGG showed considerable effectiveness against lactobacillus along with the highest bond values.

Keywords: Carbon nanoparticles; Disinfection; Methylene blue, caries affected dentin; Photodynamic therapy; Shear bond strength.

Publication types

Randomized Controlled Trial

MeSH terms

Biofilms / radiation effects
Dentin* / chemistry
Dentin* / microbiology
Dentin* / radiation effects
Disinfection* / methods
Humans
Lasers, Solid-State*
Materials Testing
Nanoparticles
Photochemotherapy / methods
Photosensitizing Agents

Substances

Photosensitizing Agents