Future Directions for Ruminant Diagnostics

John Dustin Loy; Jessie D Monday; David R Smith

doi:10.1016/j.cvfa.2022.10.008

Future Directions for Ruminant Diagnostics

Vet Clin North Am Food Anim Pract. 2023 Mar;39(1):175-183. doi: 10.1016/j.cvfa.2022.10.008.

Authors

John Dustin Loy¹, Jessie D Monday², David R Smith³

Affiliations

¹ College of Veterinary Medicine, Mississippi State University, 240 Wise Center Drive, PO Box 6100, Mississippi State, MS 39762, USA. Electronic address: jdloy@unl.edu.
² Texas A&M Veterinary Medical Diagnostic Laboratory - Canyon, WT Box 60818, 3209 Russell Long Boulevard, Canyon, TX 79016, USA.
³ College of Veterinary Medicine, Mississippi State University, 240 Wise Center Drive, PO Box 6100, Mississippi State, MS 39762, USA.

PMID: 36731997
DOI: 10.1016/j.cvfa.2022.10.008

Abstract

Diagnostic advances such as next-generation sequencing, highly multiplexed real-time PCR tests, and MALDI-TOF mass spectrometry have provided a tremendous increase in the amount of diagnostic information to clinicians. However, interpretation and application of these results to both individual and herd-level diagnostics still require the necessary skills in critical thinking and diagnostic interpretation to maximize benefit. This article provides a summary of advancements in diagnostic medicine and interpretation, as well as identifies gaps in knowledge that can be targeted to continue to build on best practices and application of diagnostic tools to improve ruminant health.

Keywords: Diagnostic error; Diagnostic interpretation; MALDI-TOF mass spectrometry; NGS; PCR; Whole genome sequencing.

Publication types

Review

MeSH terms

Animals
Disease*
High-Throughput Nucleotide Sequencing
Real-Time Polymerase Chain Reaction / veterinary
Ruminants*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / veterinary