Ultrasound-driven exercise training ameliorates degeneration of ultrasonic responses in Caenorhabditis elegans

Abstract

The inevitability of age-related degeneration makes research on degradation mitigation attractive to humans, while exercise is considered an effective means due to its powerful impact on life and health. Caenorhabditis elegans is a model animal with a short life cycle and is widely used in health and aging studies. In this work, ultrasonic stimuli in the form of surface acoustic waves (SAWs) were used to induce behavioral activities in worms. As the worms grew, ultrasound-elicited behavioral responses started to decrease in the early adulthood stage. However, this situation was significantly ameliorated when ultrasonic training sessions at an effective acoustic pressure of 1.1 MPa were performed four times per day for 5 or 7 days, while ultrasonic responses in trained nematodes were stronger than those in untrained ones. These results suggest that long-term ultrasonic training might positively intervene in aging-related degeneration. Besides, it was found that exercise driven by long-term ultrasonic training had insignificant effects on the lifespan of worms. A preliminary exploration of the neural mechanisms underlying the sensation of SAWs was also conducted. The results show that, apart from touch receptor neurons (TRNs), polymodal nociceptors FLP and PVD neurons may also be involved in the perception of ultrasound in C. elegans. The results of this study may inspire related studies on other animals or humans.

Keywords: Aging; Behavioral response; C. elegans; Degeneration; Exercise training; Ultrasound.