A pan-cancer analysis of the prognostic and immunological roles of matrix metalloprotease-1 (MMP1) in human tumors

Shuai Mao; Anliang Xia; Xuewen Tao; Dingde Ye; Jiamu Qu; Meiling Sun; Haowei Wei; Guoqiang Li

doi:10.3389/fonc.2022.1089550

A pan-cancer analysis of the prognostic and immunological roles of matrix metalloprotease-1 (MMP1) in human tumors

Front Oncol. 2023 Jan 13:12:1089550. doi: 10.3389/fonc.2022.1089550. eCollection 2022.

Authors

Shuai Mao¹, Anliang Xia¹, Xuewen Tao², Dingde Ye³, Jiamu Qu², Meiling Sun¹, Haowei Wei¹, Guoqiang Li¹

Affiliations

¹ Department of Hepatobiliary Surgery, Affiliated Drum Tower Hospital, Medical School, Nanjing University, Nanjing, China.
² Department of Hepatobiliary Surgery, Medicine School of Southeast University Nanjing Drum Tower Hospital, Nanjing, China.
³ Department of Hepatobiliary Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Abstract

Objective: Cancer remains the leading killer of human health worldwide. It has been shown that matrix metalloproteinase-1(MMP1) is related to poor prognosis in cancers such as BRCA, CESC and COAD. However, systematic pan-cancer analysis about the prognostic and immunological roles of MMP1 has not been explored. Here, the purpose of this study was to investigate the prognostic and immunological roles of MMP1 in pan-cancer and confirm cancer-promoting effect in pancreatic cancer.

Methods: In our study, bioinformatics were first used to analyze data from multiple databases. Then, several bioinformatics tools were utilized to investigate the role of MMP1 in 33 tumor types. Finally, molecular biology experiments were carried out to prove the cancer-promoting effect of MMP1 in pancreatic cancer.

Results: MMP1 expression was higher in tumor tissues than in control tissues in most tumor types. High expression of MMP1 was associated with poor overall survival (OS) and disease-free survival (DFS) in some tumor types. Further analysis of MMP1 gene mutation data showed that MMP1 mutations significantly influenced the prognosis of STAD. In addition, MMP1 expression was closely related to cancer-associated fibroblast (CAFs) infiltration in a variety of cancers and played an important role on immune infiltration score, tumor mutational burden (TMB) and microsatellite instability (MSI). Gene Ontology enrichment analysis indicated that these 20 genes were mainly related to extracellular structure organization/extracellular matrix organization/extracellular matrix disassembly/collagen metabolic process in the enriched biological processes. Finally, molecular biology experiments confirmed the cancer-promoting effect of MMP1 in pancreatic cancer.

Conclusions: Our pan-cancer analysis comprehensively proved that MMP1 expression is related with clinical prognosis and tumor immune infiltration, and MMP1 can become a prognostic and immunological biomarker.

Keywords: MMP1; immune infiltration; molecular biology experiments; pan-cancer; prognosis.

Grants and funding

This study was supported by grants from the Fifth ‘333 Project’ Scientific Research Project of Jiangsu Province (PB1736202001) and Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX22_0187).