Parabacteroides faecalis sp. nov. Isolated from Swine Faeces

Abstract

A Gram-negative, obligate anaerobic, non-motile, non-spore-forming, rod-shaped bacterial strain designated AGMB00274^T was isolated from swine faeces. An 16S rRNA gene analysis indicated that strain AGMB00274^T belonged to the genus Parabacteroides, with the highest similarity to Parabacteroides johnsonii (P. johnsonii) DSM 18315^T (sequence similarity of 94.9%). The genome size of strain AGMB00274^T was 4,308,683 bp, with a DNA G+C content of 42.5 mol%. The biochemical analysis of strain AGMB00274^T showed that it was positive for gelatin hydrolysis and α-fucosidase, but negative for the acid production from D-glucose, D-mannitol, D-maltose, salicin, glycerol, D-cellobiose, D-mannose, D-melezitose, D-sorbitol, D-trehalose, and negative for α-arabinosidase, glutamic acid decarboxylase, and pyroglutamic acid arylamidase. The dominant cellular fatty acids (> 10%) of the isolate were anteiso-C_{15: 0} (23.2%), iso-C_{15: 0} (16.6%), C_{18: 1} ω9c (16.4%), summed feature 11 (iso-C_{17: 0} 3-OH and/or C_{18: 2} DMA) (12.5%), and C_{16: 0} (11.3%). The major respiratory quinones of strain AGMB00274^T were MK-9 (55.4%) and MK-10 (44.6%). The major polar lipid was phosphatidylethanolamine. Based on phylogenetic, genetic, physiological, and chemotaxonomic analyses, as a novel species of the genus Parabacteroides, strain AGMB00274^T was proposed with the name Parabacteroides faecalis sp. nov. The type strain used was AGMB00274^T (= KCTC 25286^T = GDMCC 1.2742^T).