PLDδ, auxin, and H2O2 mediated the allelopathic effect of cycloastragenol on root growth in Arabidopsis

Abstract

Cycloastragenol (CAG) is a tetra-cyclic triterpenoid allelochemical. It has been widely studied in animals but rarely in plants. Here, we reported that a model allelochemical CAG inhibited primary root elongation of Arabidopsis by reducing the sizes of both the meristem and elongation zones. Phospholipase Dδ(PLDδ), hydrogen peroxide (H₂O₂), and auxin affected this process. After treatment with CAG, the expression of PLDδ and the activity of the Phospholipase D(PLD) enzyme increased in WT. Mutants analysis demonstrated that PLDδ negatively regulated the primary root elongation by CAG treatment. CAG treatment stimulated the accumulation of H₂O₂ in roots. The production of H₂O₂ was derived from cell wall peroxidase. Mutants analysis showed that PLDδ positively regulated the production of H₂O₂ by CAG treatment. CAG also decreased auxin content in the root tip by affecting the expression of auxin synthesis-related genes. PLDδ was involved in the auxin reduction mediated by CAG, but H₂O₂ did not participate in this process. In conclusion, PLDδ, auxin, and H₂O₂ mediated the inhibition of primary root growth by CAG in Arabidopsis.

Keywords: Arabidopsis thaliana; CAG; H(2)O(2); IAA; PLDδ; Primary root.