Effect of temperature and storage methods on liquid digestate: Focusing on the stability, phytotoxicity, and microbial community

Jing Yan; Xiaotian Chen; Ziyu Wang; ChaoJun Zhang; Xingyao Meng; Xiaoling Zhao; Xuguang Ma; Wanbin Zhu; Zongjun Cui; Xufeng Yuan

doi:10.1016/j.wasman.2023.01.023

Effect of temperature and storage methods on liquid digestate: Focusing on the stability, phytotoxicity, and microbial community

Waste Manag. 2023 Mar 15:159:1-11. doi: 10.1016/j.wasman.2023.01.023. Epub 2023 Jan 30.

Authors

Jing Yan¹, Xiaotian Chen², Ziyu Wang², ChaoJun Zhang², Xingyao Meng³, Xiaoling Zhao⁴, Xuguang Ma⁵, Wanbin Zhu², Zongjun Cui², Xufeng Yuan⁶

Affiliations

¹ Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, China; Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture and Rural Affairs/China-New Zealand Joint Laboratory for Soil Molecular Ecology, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
² Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, China.
³ Department of Environmental Science and Engineering, Beijing Technology and Business University, Beijing 100048, China.
⁴ College of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, China.
⁵ School of Chemistry, Resource and Environment, Leshan Normal University, Leshan 614000, China.
⁶ Center of Biomass Engineering, College of Agronomy and Biotechnology, China Agriculture University, Beijing 100193, China. Electronic address: ayuanxf@cau.edu.cn.

PMID: 36724571
DOI: 10.1016/j.wasman.2023.01.023

Abstract

Identifying the stability and phytotoxicity of liquid digestate (LD) is necessary for safe agricultural utilization. Storage temperature, method, and time are critical factors that affect the stability and phytotoxicity of LD. This study therefore aimed to explore the dynamics of stability, phytotoxicity, and microbial community of LD in cattle farms under different storage conditions. The results showed that the contents of solids, organic matter, nitrogen, and phosphorous decreased during storage and exhibited temperature dependency. Conversely, the seed germination index increased, which was negatively correlated with dissolved organic carbon and ammonium nitrogen and positively correlated with certain bacteria (Thermovirga and Fastidiosipila). Open storage and/or higher temperature were found to contribute to the stabilization efficiency and phytotoxicity disappearance of LD. Open storage of LD at 30 °C for 60 days and 20 °C for 90 days was safe for its agricultural utilization, while hermetic storage of LD at 30 °C for 120 days and 20 °C for 150 days was safe. However, for storage at 10 °C for 180 days, additional post-treatment is required.

Keywords: Liquid digestate; Microbial community evolution; Phytotoxicity; Stability; Structural equation model.

MeSH terms

Agriculture*
Animals
Cattle
Farms
Microbiota*
Nitrogen
Temperature

Substances

Nitrogen