Secretion of extracellular vesicles during ontogeny of the tapeworm Schistocephalus solidus

Hynek Mazanec; Nikol Buskova; Zdenko Gardian; Roman Kuchta

doi:10.14411/fp.2023.003

Secretion of extracellular vesicles during ontogeny of the tapeworm Schistocephalus solidus

Folia Parasitol (Praha). 2023 Jan 16:70:2023.003. doi: 10.14411/fp.2023.003.

Authors

Hynek Mazanec^{1

2}, Nikol Buskova², Zdenko Gardian^{1

2}, Roman Kuchta¹

Affiliations

¹ Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Ceske Budejovice, Czech Republic.
² Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic.

PMID: 36722286
DOI: 10.14411/fp.2023.003

Abstract

We provide the first ultrastructural evidence of the secretion of extracellular vesicles (EVs) across all parasitic stages of the tapeworm Schistocephalus solidus (Müller, 1776) (Cestoda: Diphyllobothriidea) using a laboratory life cycle model. We confirmed the presence of EV-like bodies in all stages examined, including the hexacanth, procercoids in the copepod, Macrocyclops albidus (Jurine, 1820), plerocercoids from the body cavity of the three-spined stickleback, Gasterosteus aculeatus Linnaeus, and adults cultivated in artificial medium. In addition, we provide description of novel tegumental structures potentially involved in EV biogenesis and the presence of unique elongated EVs similar to those previously described only in Fasciola hepatica Linnaeus, 1758 (Trematoda), Hymenolepis diminuta (Rudolphi, 1819) (Cestoda), and Trypanosoma brucei Plimmer et Bradford, 1899 (Kinetoplastida).

Keywords: : EVs; Cestoda; ESP; TEM; novel tegumental structures; ultrastructure.

MeSH terms

Animals
Cestoda*
Cestode Infections* / veterinary
Copepoda
Extracellular Vesicles*
Smegmamorpha