Camboginol and Morelloflavone from Garcinia dulcis (Roxb.) Kurz Flower Extract Promote Autophagic Cell Death against Human Glioblastoma Cells through Endoplasmic Reticulum Stress

Tanapan Siangcham; Parisa Prathaphan; Jittiporn Ruangtong; Nattaya Thongsepee; Pongsakorn Martviset; Pathanin Chantree; Phornphan Sornchuer; Kant Sangpairoj

doi:10.3746/pnf.2022.27.4.376

Camboginol and Morelloflavone from Garcinia dulcis (Roxb.) Kurz Flower Extract Promote Autophagic Cell Death against Human Glioblastoma Cells through Endoplasmic Reticulum Stress

Prev Nutr Food Sci. 2022 Dec 31;27(4):376-383. doi: 10.3746/pnf.2022.27.4.376.

Authors

Tanapan Siangcham¹, Parisa Prathaphan², Jittiporn Ruangtong², Nattaya Thongsepee^{2

3}, Pongsakorn Martviset^{2

3}, Pathanin Chantree^{2

3}, Phornphan Sornchuer^{2

3}, Kant Sangpairoj^{2

3}

Affiliations

¹ Faculty of Allied Health Sciences, Burapha University, Chonburi 20130, Thailand.
² Thammasat University Research Unit in Nutraceuticals and Food Safety, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
³ Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.

Abstract

Garcinia dulcis is a tropical plant native to Southeast Asia that is traditionally used as a folk remedy to cure several pathological symptoms. Camboginol and morelloflavone have been revealed by previous studies as the principal bioactive compounds from the flower extract of G. dulcis. The disease-preventing properties of camboginol or morelloflavone, including anti-cancer, from various parts of G. dulcis have been revealed by recent studies. Glioblastoma is the aggressive malignant stage of brain cancer and suffers from chemotherapeutic resistance. This study aimed to test the anti-cancer effect of G. dulcis flower extract against the proliferation of A172 human glioblastoma cells. The extract had cytotoxic activity and promoted cell cycle arrest at the S and G2/M phases. Autophagic cell death was promoted by cytotoxic concentrations of the extract, as observed by enhancing autophagic flux and the expression of autophagic markers. Autophagic cell death induced by the extract might be associated with endoplasmic reticulum (ER) stress. Conclusively, it was indicated by this study that the extract from the flower of G. dulcis had a protective effect against the proliferation of A172 human glioblastoma cells through the induction of ER stress-mediated cytotoxic autophagy.

Keywords: Garcinia dulcis; autophagy; endoplasmic reticulum stress; glioblastoma.